Fifty pound pigs were transported across seasons, January, April, August, and October. Measures of behavior on the truck, during the lairage, and after the transport showed some effects of increased transport stress in the continuously transported pigs (increased time spent drinking). The immune measures reflected the transport stress as well for the continuous transported group (increased white blood cell population counts and activation markers). Additionally, the microbial population measures also indicated differences in intestinal microbial populations between the two treatments, indicating that the degree of stress may be affecting this variable. Alterations in microbial populations could alter the ability of the pig to use the nutrients that it eats. Overall, in this setting of a controlled lairage environment without mixing of the pens, lairage lessened changes after transport.