The objective of this study was to calculate the inactivation of PRRSV by dose of UV254 in a “static” (i.e., virus-in-liquid solution) system. This study is the first step in evaluating the use of UV254 for the inactivation of airborne pathogens in commercial swine facilities. Viruses: The study was conducted using PRRSV isolate MN-184 (kindly provided by Dr. Scott Dee, University of Minnesota) propagated on MARC-145 cells. For comparison and contrast, a Reovirus type 3 (kindly provided by Dr. Cathy Miller, Iowa State University) grown on L929 cells was included in the experiment. Reovirus type 3 is recognized as extremely hardy and highly resistant to inactivation by UV254. Equipment: Commercially-available ultraviolet (UV254) lamps (American Ultraviolet Co., Lebanon IN) were mounted in an environmental chamber (Percival Scientific, Perry IA) capable of maintaining any pre-selected temperature between 0 and 60°C. The dose of UV254 to which the samples were exposed was measured using UV254 radiometer sensors (Technika,Co., Scottsdale AZ). No equipment was purchased through NPB #07-119.
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