In recent years increasing usage of molecular biology tools has resulted in identification of a variety of previously unknown virus species. Porcine parvovirus type 3 (PPV3) is considered an emerging pathogen in pigs although little information on its distribution and pathological potential is available. The objective of this study was to further characterize PPV3 infection in pigs. A serology assay to detect a specific antibody response to PPV3 was developed and will be a useful tool to further conduct epidemiological investigations. In addition, an infectious clone was constructed which also will be useful for future investigations. The obtained in vivo results after experimental infection of CDCD pigs indicate that PPV3 viremia is rather short and of low magnitude in healthy pigs. Furthermore, seroconversion occurred after 2 weeks which is similar to other pathogens in pigs such as classical PPV and porcine circovirus type 2 (PCV2).